Hotspot

tRNA-Gly-tufA

Genome · 100098-100156 · 59 bp

Back to Coleochaete

Pi 0.0678

MarkerSeek score 75.20

Primer available yes

Label tRNA-Gly-tufA

Feature Metadata

label_name: tRNA-Gly-tufA
feature_type: igs
region: Genome
length_bp: 59
variable_sites: 4
pi: 0.0678
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Gly-tufA_p1	GAGTTCGATTCTCGCTACCC	TCGCGAAGGAGGATTTACAA	55.0	45.0	57.9	57.0	137 bp	1.0	73.0
tRNA-Gly-tufA_p2	GAGTTCGATTCTCGCTACCC	TTCGCGAAGGAGGATTTACA	55.0	45.0	57.9	57.0	138 bp	1.0	73.0
tRNA-Gly-tufA_p3	GAGTTCGATTCTCGCTACCC	TTGTTATTCGCGAAGGAGGA	55.0	45.0	57.9	57.0	144 bp	1.0	72.9
tRNA-Gly-tufA_p4	CGAGTTCGATTCTCGCTACC	TCGCGAAGGAGGATTTACAA	55.0	45.0	58.3	57.0	138 bp	1.0	71.9
tRNA-Gly-tufA_p5	CGAGTTCGATTCTCGCTACC	TTCGCGAAGGAGGATTTACA	55.0	45.0	58.3	57.0	139 bp	1.0	71.9