Hotspot

psbZ-tRNA-Gly

Genome · 99845-100026 · 182 bp

Back to Coleochaete

Pi 0.0495

MarkerSeek score 72.80

Primer available yes

Label psbZ-tRNA-Gly

Feature Metadata

label_name: psbZ-tRNA-Gly
feature_type: igs
region: Genome
length_bp: 182
variable_sites: 9
pi: 0.0495
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-tRNA-Gly_p1	TCGCTTTGGATTGCTCTTGT	CGAACTCGCATCTTCTCCTT	45.0	50.0	58.1	57.7	294-296 bp	1.0	79.3
psbZ-tRNA-Gly_p2	TCGCTTTGGATTGCTCTTGT	AACTCGCATCTTCTCCTTGG	45.0	50.0	58.1	57.6	292-294 bp	1.0	78.5
psbZ-tRNA-Gly_p3	GCTTCTCCAGATGGATGGTC	CGAACTCGCATCTTCTCCTT	55.0	50.0	57.7	57.7	345-347 bp	1.0	77.8
psbZ-tRNA-Gly_p4	TCGCTTTGGATTGCTCTTGT	ATCGAACTCGCATCTTCTCC	45.0	50.0	58.1	57.5	296-298 bp	1.0	77.6
psbZ-tRNA-Gly_p5	TCGCTTTGGATTGCTCTTGT	TCGAACTCGCATCTTCTCCT	45.0	50.0	58.1	58.5	295-297 bp	1.0	77.3