Hotspot

tRNA-Thr-psbD

Genome · 96037-96761 · 725 bp

Back to Coleochaete

Pi 0.0345

MarkerSeek score 69.20

Primer available yes

Label tRNA-Thr-psbD

Feature Metadata

label_name: tRNA-Thr-psbD
feature_type: igs
region: Genome
length_bp: 725
variable_sites: 25
pi: 0.0345
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Thr-psbD_p1	AGTCATCGGTTCAAATCCGG	AACCGGACCAACCAACAAAT	50.0	45.0	58.0	58.2	860-873 bp	1.0	80.4
tRNA-Thr-psbD_p2	GTGGTAGAGTAACGCCATGG	AACCGGACCAACCAACAAAT	55.0	45.0	58.1	58.2	889-902 bp	1.0	80.0
tRNA-Thr-psbD_p3	TCAGTGGTAGAGTAACGCCA	AACCGGACCAACCAACAAAT	50.0	45.0	58.1	58.2	892-905 bp	1.0	79.8
tRNA-Thr-psbD_p4	AGTCATCGGTTCAAATCCGG	TAAACCGGACCAACCAACAA	50.0	45.0	58.0	57.6	862-875 bp	1.0	78.7
tRNA-Thr-psbD_p5	AGTCATCGGTTCAAATCCGG	AAACCGGACCAACCAACAAA	50.0	45.0	58.0	58.4	861-874 bp	1.0	78.6