Hotspot

tRNA-Asp-tRNA-Tyr

Genome · 95341-95513 · 173 bp

Back to Coleochaete

Pi 0.0778

MarkerSeek score 74.00

Primer available yes

Label tRNA-Asp-tRNA-Tyr

Feature Metadata

label_name: tRNA-Asp-tRNA-Tyr
feature_type: igs
region: Genome
length_bp: 173
variable_sites: 13
pi: 0.0778
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Asp-tRNA-Tyr_p1	GTATAACGGGATTGACGGGG	GTAATGCCTACGCTGGTTCA	55.0	50.0	57.8	58.0	284-290 bp	1.0	84.4
tRNA-Asp-tRNA-Tyr_p2	GTATAACGGGATTGACGGGG	TGTAATGCCTACGCTGGTTC	55.0	50.0	57.8	58.0	285-291 bp	1.0	84.4
tRNA-Asp-tRNA-Tyr_p3	GTATAACGGGATTGACGGGG	GATGTCCGAGTGGCTAAAGG	55.0	55.0	57.8	58.1	327-333 bp	1.0	83.6
tRNA-Asp-tRNA-Tyr_p4	GTATAACGGGATTGACGGGG	AGGCTTAGGACACCTCCTTT	55.0	50.0	57.8	58.0	439-445 bp	1.0	82.5
tRNA-Asp-tRNA-Tyr_p5	GTATAACGGGATTGACGGGG	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	752-760 bp	1.0	82.4