Hotspot

rpl16-rpl14

Genome · 86079-86178 · 100 bp

Back to Coleochaete

Pi 0.0694

MarkerSeek score 73.10

Primer available yes

Label rpl16-rpl14

Feature Metadata

label_name: rpl16-rpl14
feature_type: igs
region: Genome
length_bp: 100
variable_sites: 5
pi: 0.0694
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rpl14_p1	TGGAGTTTCAGAAGCAGTAGC	TCGAGCACCACTATTATCTGC	47.6	47.6	58.0	57.6	217-245 bp	1.0	66.4
rpl16-rpl14_p2	TGGAGTTTCAGAAGCAGTAGC	CGAGCACCACTATTATCTGCT	47.6	47.6	58.0	57.6	216-244 bp	1.0	66.4
rpl16-rpl14_p3	TGAGTGGAGTTTCAGAAGCAG	TCGAGCACCACTATTATCTGC	47.6	47.6	57.9	57.6	221-249 bp	1.0	65.8
rpl16-rpl14_p4	GAGTGGAGTTTCAGAAGCAGT	TCGAGCACCACTATTATCTGC	47.6	47.6	57.9	57.6	220-248 bp	1.0	65.8
rpl16-rpl14_p5	TGAGTGGAGTTTCAGAAGCAG	CGAGCACCACTATTATCTGCT	47.6	47.6	57.9	57.6	220-248 bp	1.0	65.8