Hotspot

tRNA-Ile-rpl2

Genome · 82188-82524 · 337 bp

Back to Coleochaete

Pi 0.0504

MarkerSeek score 77.40

Primer available yes

Label tRNA-Ile-rpl2

Feature Metadata

label_name: tRNA-Ile-rpl2
feature_type: igs
region: Genome
length_bp: 337
variable_sites: 17
pi: 0.0504
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ile-rpl2_p1	ATTGGAGAACTCGCAGGTTC	ATTTGTTGCGTGTACCTGGA	50.0	45.0	57.9	57.7	423 bp	1.0	80.3
tRNA-Ile-rpl2_p2	ATAAGCATCCATGGCTGAGC	ATTTGTTGCGTGTACCTGGA	50.0	45.0	58.1	57.7	464 bp	1.0	80.1
tRNA-Ile-rpl2_p3	GGTTAAGGCACCCAACTCAT	ATTTGTTGCGTGTACCTGGA	50.0	45.0	57.8	57.7	444 bp	1.0	79.2
tRNA-Ile-rpl2_p4	ATTGGAGAACTCGCAGGTTC	TTTGTTGCGTGTACCTGGAG	50.0	50.0	57.9	58.4	422 bp	1.0	79.1
tRNA-Ile-rpl2_p5	ATAAGCATCCATGGCTGAGC	TTTGTTGCGTGTACCTGGAG	50.0	50.0	58.1	58.4	463 bp	1.0	78.9