Hotspot

ndhD-tRNA-Ile

Genome · 81596-82113 · 518 bp

Back to Coleochaete

Pi 0.0402

MarkerSeek score 76.00

Primer available yes

Label ndhD-tRNA-Ile

Feature Metadata

label_name: ndhD-tRNA-Ile
feature_type: igs
region: Genome
length_bp: 518
variable_sites: 20
pi: 0.0402
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-tRNA-Ile_p1	ACTGCTTGTTCCTTAGCATCT	GAACCTGCGAGTTCTCCAAT	42.9	50.0	57.3	57.9	644-665 bp	1.0	70.5
ndhD-tRNA-Ile_p2	ACTGCTTGTTCCTTAGCATCT	ATGAGTTGGGTGCCTTAACC	42.9	50.0	57.3	57.8	623-644 bp	1.0	69.8
ndhD-tRNA-Ile_p3	ACTGCTTGTTCCTTAGCATCTT	GAACCTGCGAGTTCTCCAAT	40.9	50.0	58.0	57.9	644-665 bp	1.0	68.0
ndhD-tRNA-Ile_p4	TGCAATGTTTACTGCTTGTTCC	GAACCTGCGAGTTCTCCAAT	40.9	50.0	58.0	57.9	654-675 bp	1.0	67.9
ndhD-tRNA-Ile_p5	GCTATTCCAGGTATGAGTGGT	GAACCTGCGAGTTCTCCAAT	47.6	50.0	56.9	57.9	620-641 bp	1.0	67.4