Hotspot

tRNA-Asn-ChlL

Genome · 67159-67538 · 380 bp

Back to Coleochaete

Pi 0.0419

MarkerSeek score 73.80

Primer available yes

Label tRNA-Asn-ChlL

Feature Metadata

label_name: tRNA-Asn-ChlL
feature_type: igs
region: Genome
length_bp: 380
variable_sites: 15
pi: 0.0419
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Asn-ChlL_p1	CCCCAGGTAGGATTTGAACC	ACCACCTTTTCCATAAACTGCT	55.0	40.9	57.6	58.2	471-480 bp	1.0	65.5
tRNA-Asn-ChlL_p2	CGACAAATCGGTTAACAGCC	ACCACCTTTTCCATAAACTGCT	50.0	40.9	57.5	58.2	449-458 bp	1.0	65.1
tRNA-Asn-ChlL_p3	CCCCAGGTAGGATTTGAACC	ACCACCTTTTCCATAAACTGC	55.0	42.9	57.6	56.7	471-480 bp	1.0	64.3
tRNA-Asn-ChlL_p4	CGACAAATCGGTTAACAGCC	ACCACCTTTTCCATAAACTGC	50.0	42.9	57.5	56.7	449-458 bp	1.0	63.9
tRNA-Asn-ChlL_p5	CCCCAGGTAGGATTTGAACC	CCACCTTTTCCATAAACTGCT	55.0	42.9	57.6	56.4	470-479 bp	1.0	62.0