Hotspot

clpP

LSC · 78405-80491 · 2087 bp

Pi 0.0176

MarkerSeek score 57.30

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2087
variable_sites: 35
pi: 0.0176
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CTAGTACATGTTCCTCGGCG	AAACGGCCGGGATCATTTAA	55.0	45.0	57.9	57.9	2990 bp	0.5	58.0
clpP_p2	GGGTGGAATCCCTGCTAATC	ATACTCCAACCACCCCATGA	55.0	50.0	57.7	58.0	2841 bp	0.5	57.6
clpP_p3	GGGTGGAATCCCTGCTAATC	TGATACTCCAACCACCCCAT	55.0	50.0	57.7	58.0	2843 bp	0.5	57.6
clpP_p4	CTAGTACATGTTCCTCGGCG	ACCAAGGCAAACCCATGAAA	55.0	45.0	57.9	58.2	2950 bp	0.5	57.4
clpP_p5	GGGTGGAATCCCTGCTAATC	ACAGGGTCAGAGGGATCAAA	55.0	50.0	57.7	58.0	2754 bp	0.5	57.4