Hotspot

accD

LSC · 64936-66522 · 1587 bp

Back to Cojoba

Pi 0.0101
MarkerSeek score 54.70
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
LSC
length_bp
1587
variable_sites
16
pi
0.0101
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 AATAGAGGCAAGAAAGCTCT TGAATAATTTAAAGCTCTACTTCA 40.0 25.0 54.0 52.2 1632-1917 bp 1.0 40.7
accD_p2 TGTAAATAGAGGCAAGAAAGCTCT TGAATAATTTAAAGCTCTACTTCA 37.5 25.0 57.9 52.2 1636-1921 bp 1.0 40.7
accD_p3 AAATAGAGGCAAGAAAGCTCT TGAATAATTTAAAGCTCTACTTCA 38.1 25.0 54.9 52.2 1633-1918 bp 1.0 40.7
accD_p4 TGTAAATAGAGGCAAGAAAGCTC TGAATAATTTAAAGCTCTACTTCA 39.1 25.0 56.6 52.2 1636-1921 bp 1.0 40.7
accD_p5 TGTAAATAGAGGCAAGAAAGCT TGAATAATTTAAAGCTCTACTTCA 36.4 25.0 55.4 52.2 1636-1921 bp 1.0 40.7