Hotspot

rpl23-rpl2

IRa · 175994-176011 · 18 bp

Back to Cojoba

Pi 0.0556
MarkerSeek score 81.90
Primer available yes
Label rpl23-rpl2

Feature Metadata

label_name
rpl23-rpl2
feature_type
igs
region
IRa
length_bp
18
variable_sites
1
pi
0.0556
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-rpl2_p1 GGTAGAAGGATGGGGCCTAT CACTTGACTGTCTACGGCTC 55.0 55.0 58.0 58.0 192 bp 1.0 79.9
rpl23-rpl2_p2 GGTAGAAGGATGGGGCCTAT CCACAACGATGCTGTCCATA 55.0 50.0 58.0 58.0 239 bp 1.0 79.8
rpl23-rpl2_p3 GGTAGAAGGATGGGGCCTAT GACTGTCTACGGCTCCATTG 55.0 55.0 58.0 58.1 187 bp 1.0 79.5
rpl23-rpl2_p4 CCCGGTAAAGGGTAGAAGGA CACTTGACTGTCTACGGCTC 55.0 55.0 58.1 58.0 202 bp 1.0 79.2
rpl23-rpl2_p5 CCCGGTAAAGGGTAGAAGGA CCACAACGATGCTGTCCATA 55.0 50.0 58.1 58.0 249 bp 1.0 79.1