Hotspot

clpP-psbB

LSC · 80492-80984 · 493 bp

Back to Cojoba

Pi 0.0226
MarkerSeek score 59.20
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
LSC
length_bp
493
variable_sites
11
pi
0.0226
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 TTTGGAACACCAACAGGCAT TGGACAGAAAGTAAACGGCC 45.0 50.0 58.2 58.1 574-581 bp 1.0 79.9
clpP-psbB_p2 CTTTTGGAACACCAACAGGC TGGACAGAAAGTAAACGGCC 50.0 50.0 57.8 58.1 576-583 bp 1.0 79.8
clpP-psbB_p3 TTTGGAACACCAACAGGCAT AAACGGCCGGGATCATTTAA 45.0 45.0 58.2 57.9 562-569 bp 1.0 79.8
clpP-psbB_p4 CTTTTGGAACACCAACAGGC AAACGGCCGGGATCATTTAA 50.0 45.0 57.8 57.9 564-571 bp 1.0 79.7
clpP-psbB_p5 TTTGGAACACCAACAGGCAT CGATACCAAGGCAAACCCAT 45.0 50.0 58.2 58.2 526-533 bp 1.0 79.1