Hotspot

rps12-clpP

LSC · 78158-78404 · 247 bp

Back to Cojoba

Pi 0.0648
MarkerSeek score 68.80
Primer available yes
Label rps12-clpP

Feature Metadata

label_name
rps12-clpP
feature_type
igs
region
LSC
length_bp
247
variable_sites
16
pi
0.0648
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-clpP_p1 TGCACCAACGGAAACCATAA GAGCCTATTTCAGCGTCACA 45.0 50.0 58.0 58.0 1274-1565 bp 1.0 85.2
rps12-clpP_p2 TTGCACCAACGGAAACCATA GAGCCTATTTCAGCGTCACA 45.0 50.0 58.0 58.0 1275-1566 bp 1.0 85.2
rps12-clpP_p3 CCCTCTTAGTCCGTTCTTGC GAGCCTATTTCAGCGTCACA 55.0 50.0 58.0 58.0 1421-1712 bp 1.0 85.1
rps12-clpP_p4 GTGAAGATTAGCTGGGGAGC GAGCCTATTTCAGCGTCACA 55.0 50.0 58.0 58.0 1444-1735 bp 1.0 84.9
rps12-clpP_p5 ACTTCTTAAACGGCGAGGTC GAGCCTATTTCAGCGTCACA 50.0 50.0 57.9 58.0 1613-1904 bp 1.0 84.4