Hotspot

ndhC-trnV-UAC

LSC · 57488-58509 · 1022 bp

Back to Cojoba

Pi 0.012
MarkerSeek score 58.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1022
variable_sites
12
pi
0.012
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCGGCCCTTTATTAATCGGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.5 57.8 1170-1181 bp 1.0 76.8
ndhC-trnV-UAC_p2 CGGCCCTTTATTAATCGGGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.5 57.8 1169-1180 bp 1.0 76.8
ndhC-trnV-UAC_p3 TCCGGCCCTTTATTAATCGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.4 57.8 1171-1182 bp 1.0 76.1
ndhC-trnV-UAC_p4 CCGGCCCTTTATTAATCGGG GAAGGTCTACGGTTCGAGTC 55.0 55.0 58.5 57.5 1169-1180 bp 1.0 74.0
ndhC-trnV-UAC_p5 CGGCCCTTTATTAATCGGGG GAAGGTCTACGGTTCGAGTC 55.0 55.0 58.5 57.5 1168-1179 bp 1.0 74.0