Hotspot

psbI-trnS-GCU

LSC · 8996-9431 · 436 bp

Back to Cojoba

Pi 0.026
MarkerSeek score 69.20
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
436
variable_sites
11
pi
0.026
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 CCTATCTAACGATCCAGGACG TGGACTAAAGCGTCGGATTG 52.4 50.0 57.2 58.0 539-552 bp 1.0 68.8
psbI-trnS-GCU_p2 CCTATCTAACGATCCAGGACG GTGGACTAAAGCGTCGGATT 52.4 50.0 57.2 58.0 540-553 bp 1.0 68.8
psbI-trnS-GCU_p3 CCTATCTAACGATCCAGGACG TTTTTCGTACCGAGGGTTCG 52.4 50.0 57.2 58.2 502-515 bp 1.0 67.4
psbI-trnS-GCU_p4 CCTATCTAACGATCCAGGACG CAATTGGGGAGAGATGGCTG 52.4 55.0 57.2 58.3 561-574 bp 1.0 66.2
psbI-trnS-GCU_p5 CCTATCTAACGATCCAGGACG GACTAAAGCGTCGGATTGCT 52.4 50.0 57.2 58.4 537-550 bp 1.0 66.0