Hotspot

rpl22

LSC · 82289-82735 · 447 bp

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Pi 0.0123
MarkerSeek score 36.90
Primer available yes
Label rpl22

Feature Metadata

label_name
rpl22
feature_type
gene
region
LSC
length_bp
447
variable_sites
11
pi
0.0123
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
36.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22_p1 ACCAAGTCTGAAACCGAGTG GGTAGGCGGGTAATAACCTT 50.0 50.0 57.8 56.7 569 bp 1.0 69.1
rpl22_p2 CCAAGTCTGAAACCGAGTGG GGTAGGCGGGTAATAACCTT 55.0 50.0 58.5 56.7 568 bp 1.0 67.2
rpl22_p3 CAAGTCTGAAACCGAGTGGA GGTAGGCGGGTAATAACCTT 50.0 50.0 57.5 56.7 567 bp 1.0 66.9
rpl22_p4 CAAGTCTGAAACCGAGTGGAT GGTAGGCGGGTAATAACCTT 47.6 50.0 58.0 56.7 567 bp 1.0 62.7
rpl22_p5 TGGTGATTTTGGGTTGTACCA GGTAGGCGGGTAATAACCTT 42.9 50.0 57.7 56.7 586 bp 1.0 60.5