Hotspot

psbE-petL

LSC · 64497-65757 · 1261 bp

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Pi 0.000397
MarkerSeek score 45.00
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1261
variable_sites
1
pi
0.000397
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 TGACAAATAACCAACCCGCA AAGCAGCCAGTAGAAAACCG 45.0 50.0 58.0 58.5 1419-1421 bp 1.0 76.1
psbE-petL_p2 TGACAAATAACCAACCCGCA GCAGCCAGTAGAAAACCGAA 45.0 50.0 58.0 58.5 1417-1419 bp 1.0 76.1
psbE-petL_p3 CAACCCGCAATGAATAGGGA AAGCAGCCAGTAGAAAACCG 50.0 50.0 57.9 58.5 1408-1410 bp 1.0 75.8
psbE-petL_p4 CAACCCGCAATGAATAGGGA GCAGCCAGTAGAAAACCGAA 50.0 50.0 57.9 58.5 1406-1408 bp 1.0 75.8
psbE-petL_p5 ACCAACCCGCAATGAATAGG AAGCAGCCAGTAGAAAACCG 50.0 50.0 58.2 58.5 1410-1412 bp 1.0 74.3