Hotspot

rbcL-rpl23

LSC · 58611-58899 · 289 bp

Back to Coix

Pi 0.0104
MarkerSeek score 31.80
Primer available yes
Label rbcL-rpl23

Feature Metadata

label_name
rbcL-rpl23
feature_type
igs
region
LSC
length_bp
289
variable_sites
6
pi
0.0104
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
31.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-rpl23_p1 GGCTTCAGGGGGTATTCATG GTCATTGCAATTGCCGGAAA 55.0 45.0 58.0 57.9 1312 bp 1.0 80.6
rbcL-rpl23_p2 TCTCATTATTGCCGCGACAA GTCATTGCAATTGCCGGAAA 45.0 45.0 58.0 57.9 1602 bp 1.0 80.3
rbcL-rpl23_p3 TTGTCTCATTATTGCCGCGA GTCATTGCAATTGCCGGAAA 45.0 45.0 58.0 57.9 1605 bp 1.0 80.3
rbcL-rpl23_p4 GGCTTCAGGGGGTATTCATG AGAAGAGTTCGACCCAATGC 55.0 50.0 58.0 57.9 714 bp 1.0 80.1
rbcL-rpl23_p5 GGCTTCAGGGGGTATTCATG GAAGAGTTCGACCCAATGCT 55.0 50.0 58.0 57.9 713 bp 1.0 80.1