Hotspot

ndhC-trnV-UAC

LSC · 52442-53380 · 939 bp

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Pi 0.000532
MarkerSeek score 45.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
939
variable_sites
1
pi
0.000532
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTAACCGGGGCTAAAAGTCC GGTTCGAATCCGTATAGCCC 55.0 55.0 58.0 57.9 1065 bp 1.0 79.2
ndhC-trnV-UAC_p2 CTAACCGGGGCTAAAAGTCC AGCCCAATGCAGAAAAATGC 55.0 45.0 58.0 57.9 1158 bp 1.0 79.0
ndhC-trnV-UAC_p3 CTAACCGGGGCTAAAAGTCC CGGTTCGAATCCGTATAGCC 55.0 55.0 58.0 58.3 1066 bp 1.0 77.4
ndhC-trnV-UAC_p4 TAACCGGGGCTAAAAGTCCT GGTTCGAATCCGTATAGCCC 50.0 55.0 58.3 57.9 1064 bp 1.0 76.7
ndhC-trnV-UAC_p5 TAACCGGGGCTAAAAGTCCT AGCCCAATGCAGAAAAATGC 50.0 45.0 58.3 57.9 1157 bp 1.0 76.5