Hotspot

psaA-ycf3

LSC · 43865-44474 · 610 bp

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Pi 0.00082
MarkerSeek score 45.30
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
610
variable_sites
1
pi
0.00082
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GGGGCCCTTAGCTATTGTTC TGGTTTGATCAAGCTGCTGA 55.0 45.0 58.0 57.7 841 bp 1.0 77.8
psaA-ycf3_p2 GTCTGGCCCATTCCTCAAAA TGGTTTGATCAAGCTGCTGA 50.0 45.0 58.1 57.7 806 bp 1.0 77.3
psaA-ycf3_p3 GGGGCCCTTAGCTATTGTTC GCTATAGCGCTTACTCCAGG 55.0 55.0 58.0 57.6 808 bp 1.0 77.1
psaA-ycf3_p4 GTCTGGCCCATTCCTCAAAA GCTATAGCGCTTACTCCAGG 50.0 55.0 58.1 57.6 773 bp 1.0 76.6
psaA-ycf3_p5 GGTTCCGACGGACGAATAAT TGGTTTGATCAAGCTGCTGA 50.0 45.0 57.8 57.7 744 bp 1.0 76.2