Hotspot

psbM-petN

LSC · 18756-19555 · 800 bp

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Pi 0.005
MarkerSeek score 48.20
Primer available yes
Label psbM-petN

Feature Metadata

label_name
psbM-petN
feature_type
igs
region
LSC
length_bp
800
variable_sites
8
pi
0.005
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-petN_p1 TGTTCATTCTAGTTCCTACTGCC TAGTAAGTCTCGCTTGGGCT 43.5 50.0 57.9 58.2 951 bp 1.0 54.8
psbM-petN_p2 TGTTCATTCTAGTTCCTACTGCC GTAGTATGGGGGAGGAGTGG 43.5 60.0 57.9 58.3 895 bp 1.0 53.8
psbM-petN_p3 TGTTCATTCTAGTTCCTACTGCC TAGTATGGGGGAGGAGTGGA 43.5 55.0 57.9 58.4 894 bp 1.0 53.0
psbM-petN_p4 GTTCATTCTAGTTCCTACTGCCT TAGTAAGTCTCGCTTGGGCT 43.5 50.0 57.7 58.2 950 bp 1.0 52.7
psbM-petN_p5 TGTTCATTCTAGTTCCTACTGCC CGCTAGTAGTATGGGGGAGG 43.5 60.0 57.9 58.5 900 bp 1.0 52.4