Hotspot

rpoC1

LSC · 21172-23972 · 2801 bp

Pi 0.00301

MarkerSeek score 55.60

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2801
variable_sites: 25
pi: 0.00301
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	AATCCTAGCTGAACCGGAGA	CTCGTTCGAGAACTACGATCC	50.0	52.4	57.8	58.0	2957-2970 bp	1.0	71.5
rpoC1_p2	AATCCTAGCTGAACCGGAGA	TCGTTCGAGAACTACGATCC	50.0	50.0	57.8	56.9	2956-2969 bp	1.0	70.9
rpoC1_p3	AATCCTAGCTGAACCGGAGA	CGTTCGAGAACTACGATCCT	50.0	50.0	57.8	56.9	2955-2968 bp	1.0	70.8
rpoC1_p4	ATCCTAGCTGAACCGGAGAG	CTCGTTCGAGAACTACGATCC	55.0	52.4	58.3	58.0	2956-2969 bp	1.0	70.4
rpoC1_p5	AATCCTAGCTGAACCGGAGA	CCGGATTCATAGGAAGGAAGC	50.0	52.4	57.8	58.2	2892-2905 bp	1.0	69.9