Hotspot

rpoA

LSC · 78657-79664 · 1008 bp

Back to Coffea

Pi 0.00325
MarkerSeek score 54.90
Primer available yes
Label rpoA

Feature Metadata

label_name
rpoA
feature_type
gene
region
LSC
length_bp
1008
variable_sites
12
pi
0.00325
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoA_p1 ACGATTCAAAACCTATTTTCATCT AATGGCTGTAGACCTCCCAA 29.2 50.0 55.1 58.3 1183-1184 bp 1.0 40.9
rpoA_p2 ACGATTCAAAACCTATTTTCATCT ATGGCTGTAGACCTCCCAAA 29.2 50.0 55.1 58.3 1182-1183 bp 1.0 40.9
rpoA_p3 ACACATATTCAATTCTGACTCT AATGGCTGTAGACCTCCCAA 31.8 50.0 52.9 58.3 1236-1267 bp 1.0 40.9
rpoA_p4 ACACATATTCAATTCTGACTCT ATGGCTGTAGACCTCCCAAA 31.8 50.0 52.9 58.3 1235-1266 bp 1.0 40.9
rpoA_p5 ACGATTCAAAACCTATTTTCATCT TGGCTGTAGACCTCCCAAAA 29.2 50.0 55.1 58.6 1181-1182 bp 1.0 40.9