Hotspot

psbA

LSC · 304-1365 · 1062 bp

Pi 0.00123

MarkerSeek score 29.80

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 5
pi: 0.00123
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GATCCACTTGGCTACATCCG	CCTCACGTCGGAAGAAGAAG	55.0	55.0	58.1	58.0	1942-1972 bp	1.0	82.2
psbA_p2	CAATCCACTGCCTTGATCCA	CCTCACGTCGGAAGAAGAAG	50.0	55.0	57.9	58.0	1956-1986 bp	1.0	82.1
psbA_p3	AATCCACTGCCTTGATCCAC	CCTCACGTCGGAAGAAGAAG	50.0	55.0	57.9	58.0	1955-1985 bp	1.0	82.1
psbA_p4	ACAATCCACTGCCTTGATCC	CCTCACGTCGGAAGAAGAAG	50.0	55.0	57.9	58.0	1957-1987 bp	1.0	82.1
psbA_p5	CATCCGCCCCTCTACTCTAT	CCTCACGTCGGAAGAAGAAG	55.0	55.0	57.8	58.0	1928-1958 bp	1.0	81.4