Hotspot

petD

LSC · 77278-78477 · 1200 bp

Back to Coffea

Pi 0.0063
MarkerSeek score 46.30
Primer available yes
Label petD

Feature Metadata

label_name
petD
feature_type
gene
region
LSC
length_bp
1200
variable_sites
24
pi
0.0063
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD_p1 GTGGGTCGAAGGGAATTCTC ACTTTGAAGCGACTATTCCCT 55.0 42.9 58.0 57.0 1282-1290 bp 1.0 65.8
petD_p2 GTGGGTCGAAGGGAATTCTC GGGAGAATTCACTTTGAAGCG 55.0 47.6 58.0 57.5 1292-1300 bp 0.909091 65.2
petD_p3 TGGGTCGAAGGGAATTCTCT GGGAGAATTCACTTTGAAGCG 50.0 47.6 58.0 57.5 1291-1299 bp 0.909091 65.0
petD_p4 CTGTGGGTCGAAGGGAATTC GGGAGAATTCACTTTGAAGCG 55.0 47.6 58.3 57.5 1294-1302 bp 0.909091 63.3
petD_p5 GGGTCGAAGGGAATTCTCTG GGGAGAATTCACTTTGAAGCG 55.0 47.6 57.7 57.5 1290-1298 bp 0.909091 62.9