Hotspot

clpP1

LSC · 70735-72736 · 2002 bp

Pi 0.00552

MarkerSeek score 45.80

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2002
variable_sites: 29
pi: 0.00552
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	TCCTTGGCTCTACCCATGAA	AACAATACGCAATGGGGGTT	50.0	45.0	58.0	58.1	2913-2934 bp	1.0	81.6
clpP1_p2	AGTTCATCCTCCTTGGCTCT	AACAATACGCAATGGGGGTT	50.0	45.0	58.0	58.1	2922-2943 bp	1.0	81.6
clpP1_p3	TAGCTGACCCTCTTAGTCCG	AACAATACGCAATGGGGGTT	55.0	45.0	57.9	58.1	2819-2840 bp	1.0	81.6
clpP1_p4	ACTTTTGTGCCTGCTCATGA	AACAATACGCAATGGGGGTT	45.0	45.0	58.0	58.1	2548-2570 bp	1.0	81.4
clpP1_p5	AAATGGAGTCCCCTCTTCCA	AACAATACGCAATGGGGGTT	50.0	45.0	57.9	58.1	2624-2639 bp	0.727273	69.5