Hotspot

rpl32-trnL-UAG

SSC · 113959-114807 · 849 bp

Back to Coffea

Pi 0.0109
MarkerSeek score 42.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
849
variable_sites
26
pi
0.0109
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGAAGGGATATTGGACAGCC TCCTAAGAGCAGCGTGTCTA 55.0 50.0 57.7 57.9 974-999 bp 1.0 80.9
rpl32-trnL-UAG_p2 GGAAGGGATATTGGACAGCC CCGAATAAGTCCAACGCGTA 55.0 50.0 57.7 58.1 1296-1321 bp 1.0 80.4
rpl32-trnL-UAG_p3 GGAAGGGATATTGGACAGCC AGCAGCGTGTCTACCAATTT 55.0 45.0 57.7 57.8 967-992 bp 1.0 80.4
rpl32-trnL-UAG_p4 GGAAGGGATATTGGACAGCC CTAAGAGCAGCGTGTCTACC 55.0 55.0 57.7 57.8 972-997 bp 1.0 80.3
rpl32-trnL-UAG_p5 GGAGGAAGTACCCGAGTTTG CCGAATAAGTCCAACGCGTA 55.0 50.0 57.6 58.1 1936-1961 bp 0.727273 67.9