Hotspot

rpl20-rps12

LSC · 69706-70475 · 770 bp

Back to Coffea

Pi 0.00789
MarkerSeek score 64.70
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
770
variable_sites
19
pi
0.00789
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TCGCGTAAGTCTTGAATGGG GAGGAACATGTACTCGGGTG 50.0 55.0 58.0 57.7 881-899 bp 1.0 80.9
rpl20-rps12_p2 TCGCGTAAGTCTTGAATGGG AGGAACATGTACTCGGGTGT 50.0 50.0 58.0 58.4 880-898 bp 1.0 80.4
rpl20-rps12_p3 TCGCGTAAGTCTTGAATGGG GCGTCGAGGAACATGTACTC 50.0 55.0 58.0 58.5 886-904 bp 1.0 79.6
rpl20-rps12_p4 GTAAGTCTTGAATGGGCCCC GAGGAACATGTACTCGGGTG 55.0 55.0 58.2 57.7 877-895 bp 1.0 79.1
rpl20-rps12_p5 TCGCGTAAGTCTTGAATGGG AAATGTCACGAAATCCCCCG 50.0 50.0 58.0 58.5 925-943 bp 1.0 78.9