Hotspot

accD-psaI

LSC · 59331-60081 · 751 bp

Back to Coffea

Pi 0.0149
MarkerSeek score 55.40
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
751
variable_sites
29
pi
0.0149
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 AGTTCCATGCTTTCGTTCCT CCATTGCAATTGCCGGAAAT 45.0 45.0 57.4 58.0 866-877 bp 1.0 78.9
accD-psaI_p2 AGTTCCATGCTTTCGTTCCT AAGAAGCCATTGCAATTGCC 45.0 45.0 57.4 57.9 872-883 bp 1.0 78.3
accD-psaI_p3 AGTTCCATGCTTTCGTTCCT GCCGGAAATACTAGGCCTAC 45.0 55.0 57.4 57.5 855-866 bp 1.0 75.6
accD-psaI_p4 AGTTCCATGCTTTCGTTCCTT CCATTGCAATTGCCGGAAAT 42.9 45.0 58.1 58.0 866-877 bp 1.0 74.4
accD-psaI_p5 TCCATGCTTTCGTTCCTTTGA CCATTGCAATTGCCGGAAAT 42.9 45.0 58.1 58.0 863-874 bp 1.0 74.3