Hotspot

ndhC-trnV-UAC

LSC · 50717-51896 · 1180 bp

Back to Coffea

Pi 0.00748
MarkerSeek score 54.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1180
variable_sites
37
pi
0.00748
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 ATCATATTCGTAAAGAAGAAACAT GTTCGAGTCCGTATAGCCCT 25.0 55.0 52.6 58.7 1094-1260 bp 1.0 46.5
ndhC-trnV-UAC_p2 ATCATATTCGTAAAGAAGAAACAT AGAAGGTCTACGGTTCGAGT 25.0 50.0 52.6 57.8 1106-1272 bp 1.0 46.4
ndhC-trnV-UAC_p3 AGACTTGATATTATTAGAAATGTCCA AGAAGGTCTACGGTTCGAGT 26.9 50.0 54.3 57.8 1138-1304 bp 1.0 46.4
ndhC-trnV-UAC_p4 ATCATATTCGTAAAGAAGAAACAT GAAGGTCTACGGTTCGAGTC 25.0 55.0 52.6 57.5 1105-1271 bp 1.0 46.4
ndhC-trnV-UAC_p5 TATCATATTCGTAAAGAAGAAACA AGAAGGTCTACGGTTCGAGT 25.0 50.0 52.1 57.8 1107-1273 bp 1.0 46.4