Hotspot

psbM-trnD-GUC

LSC · 30019-30938 · 920 bp

Back to Coffea

Pi 0.0111
MarkerSeek score 57.20
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
920
variable_sites
26
pi
0.0111
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGGAACTAGAATGAATAGTGCAGT TTCAATTGGTCAGAGCACCG 37.5 50.0 57.4 58.5 1027-1042 bp 1.0 43.6
psbM-trnD-GUC_p2 GGAACTAGAATGAATAGTGCAGT TTCAATTGGTCAGAGCACCG 39.1 50.0 56.1 58.5 1026-1041 bp 1.0 43.6
psbM-trnD-GUC_p3 CGTAGGAACTAGAATGAATAGTGC TTCAATTGGTCAGAGCACCG 41.7 50.0 57.0 58.5 1030-1045 bp 1.0 43.5
psbM-trnD-GUC_p4 TTGACTGACTGTTTTTACGT TTCAATTGGTCAGAGCACCG 35.0 50.0 53.0 58.5 1069-1084 bp 1.0 43.4
psbM-trnD-GUC_p5 TTTGACTGACTGTTTTTACGT TTCAATTGGTCAGAGCACCG 33.3 50.0 53.9 58.5 1070-1085 bp 1.0 43.4