Hotspot

atpH-atpI

LSC · 13914-14960 · 1047 bp

Back to Coffea

Pi 0.00821
MarkerSeek score 62.60
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1047
variable_sites
40
pi
0.00821
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT TTTTTGCAACTTTAGCCGCG 45.0 45.0 58.0 58.5 1134-1143 bp 1.0 78.7
atpH-atpI_p2 CAGCAGTTCCTTGACCAACT TTTTTGCAACTTTAGCCGCG 50.0 45.0 58.0 58.5 1196-1205 bp 1.0 78.6
atpH-atpI_p3 AGCCAATCCAGCAGCAATAA TTTTTGCAACTTTAGCCGCG 45.0 45.0 57.9 58.5 1150-1159 bp 1.0 77.8
atpH-atpI_p4 AATAACGGAAGCGGCAGAAA TTTTTGCAACTTTAGCCGCG 45.0 45.0 58.2 58.5 1135-1144 bp 1.0 77.5
atpH-atpI_p5 GCCAATCCAGCAGCAATAAC TTTTTGCAACTTTAGCCGCG 50.0 45.0 57.8 58.5 1149-1158 bp 1.0 77.3