Hotspot

ycf1

SSC · 128877-134408 · 5532 bp

Back to Coelogyne

Pi 0.0238

MarkerSeek score 67.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5532
variable_sites: 1151
pi: 0.0238
unique_haplotype_count: 51
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTTCGGGGAGAACCAAAGG	TCGGATTTTCGTCGCAATCT	50.0	45.0	58.0	58.0	80-732 bp	0.846154	83.7
ycf1_p2	AAAAAGGTCGTTTTCGGGGA	TCGGATTTTCGTCGCAATCT	45.0	45.0	57.9	58.0	712-2278 bp	0.769231	80.1
ycf1_p3	ATCCTCATCCCACTCGGTAG	TCGGATTTTCGTCGCAATCT	55.0	45.0	58.0	58.0	441-2279 bp	0.75	77.9
ycf1_p4	CCTCATCCCACTCGGTAGAT	TCGGATTTTCGTCGCAATCT	55.0	45.0	58.0	58.0	439-2278 bp	0.75	77.9
ycf1_p5	GAGAACCAAAGGGAAGCTCC	TCGGATTTTCGTCGCAATCT	55.0	45.0	58.2	58.0	694-2279 bp	0.692308	75.6