Hotspot

psaC-ndhE

SSC · 122041-122928 · 888 bp

Back to Coelogyne

Pi 0.0342

MarkerSeek score 87.60

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 888
variable_sites: 237
pi: 0.0342
unique_haplotype_count: 50
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 87.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	AGCTCGAACACATTGGGTAC	CTGAAGCAGCTATTGGGCTA	50.0	50.0	57.9	57.4	842-2277 bp	0.980769	85.5
psaC-ndhE_p2	AGCTCGAACACATTGGGTAC	TGAAGCAGCTATTGGGCTAG	50.0	50.0	57.9	57.4	841-2278 bp	0.980769	85.5
psaC-ndhE_p3	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	50.0	50.0	58.1	57.4	884-2277 bp	0.980769	85.2
psaC-ndhE_p4	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	50.0	50.0	58.1	57.4	883-2278 bp	0.980769	85.2
psaC-ndhE_p5	GCTCGAACACATTGGGTACA	TGAAGCAGCTATTGGGCTAG	50.0	50.0	58.2	57.4	840-2279 bp	0.980769	84.6