Hotspot

ndhF-rpl32

SSC · 116959-117940 · 982 bp

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Pi 0.0373

MarkerSeek score 78.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 982
variable_sites: 276
pi: 0.0373
unique_haplotype_count: 49
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGCCGAAAACCACATAGCTA	CTTTTTGACTGTCCGGTGGA	50.0	50.0	58.0	58.0	80-1354 bp	1.0	91.4
ndhF-rpl32_p2	GGCCGAAAACCACATAGCTA	TCGAGACCCAACACCTTAGT	50.0	50.0	58.0	58.0	1424-2279 bp	0.961538	90.2
ndhF-rpl32_p3	AGATTGGCCGAAAACCACAT	TCGAGACCCAACACCTTAGT	45.0	50.0	58.1	58.0	1429-2278 bp	0.961538	89.8
ndhF-rpl32_p4	AGCAACTGCACCGACAAATA	TCGAGACCCAACACCTTAGT	45.0	50.0	58.1	58.0	1973-2358 bp	0.961538	89.5
ndhF-rpl32_p5	TAGCAACTGCACCGACAAAT	TCGAGACCCAACACCTTAGT	45.0	50.0	58.1	58.0	1974-2359 bp	0.961538	89.5