Hotspot

psbB-psbT

LSC · 77240-77714 · 475 bp

Back to Coelogyne

Pi 0.0453

MarkerSeek score 81.20

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 475
variable_sites: 127
pi: 0.0453
unique_haplotype_count: 49
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TGGAGATCCAACTACAAGGAGA	GGAATGTATAAACCAATGCTTCCA	45.5	37.5	57.9	57.5	462-642 bp	1.0	52.0
psbB-psbT_p2	TGGAGATCCAACTACAAGGAGA	TCCTAAAGTCGAAACTAAGAGGA	45.5	39.1	57.9	56.4	482-662 bp	1.0	52.0
psbB-psbT_p3	TGGAGATCCAACTACAAGGAGA	AGTCGAAACTAAGAGGAATGT	45.5	38.1	57.9	54.3	476-656 bp	1.0	52.0
psbB-psbT_p4	TGGAGATCCAACTACAAGGAGA	AGGAATGTATAAACCAATGCTTCC	45.5	37.5	57.9	57.3	463-643 bp	1.0	52.0
psbB-psbT_p5	GGAGATCCAACTACAAGGAGAC	GGAATGTATAAACCAATGCTTCCA	50.0	37.5	57.6	57.5	461-641 bp	1.0	52.0