Hotspot

psbE-petL

LSC · 67318-68487 · 1170 bp

Back to Coelogyne

Pi 0.0143

MarkerSeek score 75.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1170
variable_sites: 152
pi: 0.0143
unique_haplotype_count: 49
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GCTGGGGTTATAGTTGAAGCA	50.0	47.6	57.9	58.0	1105-1364 bp	1.0	83.6
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GGGTTATAGTTGAAGCAGCCA	50.0	47.6	57.9	58.0	1101-1360 bp	1.0	83.6
psbE-petL_p3	CAACCCGCAATGAATAGGGA	TGGGGTTATAGTTGAAGCAGC	50.0	47.6	57.9	58.0	1103-1362 bp	1.0	83.6
psbE-petL_p4	ACTGACGAATAACCAACCCG	GGGTTATAGTTGAAGCAGCCA	50.0	47.6	57.9	58.0	1114-1373 bp	1.0	83.3
psbE-petL_p5	ACTGACGAATAACCAACCCG	TGGGGTTATAGTTGAAGCAGC	50.0	47.6	57.9	58.0	1116-1375 bp	1.0	83.3