Hotspot

atpF-atpH

LSC · 13684-13840 · 157 bp

Back to Coelogyne

Pi 0.0206

MarkerSeek score 74.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 157
variable_sites: 29
pi: 0.0206
unique_haplotype_count: 46
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TAAACTCGAAACTCCCAGCG	ATTTATGGACTGGTCGTGGC	50.0	50.0	57.9	58.0	251-292 bp	1.0	91.1
atpF-atpH_p2	CCCGCGGAAACAAAAGAATC	ATTTATGGACTGGTCGTGGC	50.0	50.0	58.0	58.0	80-260 bp	0.980769	90.7
atpF-atpH_p3	TAAACTCGAAACTCCCAGCG	GGTCGTGGCATTAGCTCTTT	50.0	50.0	57.9	58.3	240-281 bp	1.0	89.3
atpF-atpH_p4	CCCGCGGAAACAAAAGAATC	GGTCGTGGCATTAGCTCTTT	50.0	50.0	58.0	58.3	208-2278 bp	0.980769	89.0
atpF-atpH_p5	CGCGGAAACAAAAGAATCGG	ATTTATGGACTGGTCGTGGC	50.0	50.0	58.4	58.0	80-258 bp	0.961538	87.0