Hotspot

rps16-trnQ-UUG

LSC · 6096-7216 · 1121 bp

Back to Coelogyne

Pi 0.0298
MarkerSeek score 79.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1121
variable_sites
272
pi
0.0298
unique_haplotype_count
52
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GACCAAATCAAAATGGGGCG 45.0 50.0 58.1 58.0 1005-1301 bp 1.0 90.8
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 944-1240 bp 1.0 90.3
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 948-1244 bp 1.0 89.2
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA CAAATCAAAATGGGGCGTGG 45.0 50.0 58.1 58.3 1002-1298 bp 1.0 88.7
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA CCAAATCAAAATGGGGCGTG 45.0 50.0 58.1 58.3 1003-1299 bp 1.0 88.7