Hotspot

matK-trnK-UUU

LSC · 3275-4361 · 1087 bp

Back to Coelogyne

Pi 0.0265

MarkerSeek score 72.80

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 1087
variable_sites: 224
pi: 0.0265
unique_haplotype_count: 50
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TCGCGACGAGAATGGAATTT	ATCCGGGTTGCTAACTCAAC	45.0	50.0	58.0	57.9	1341-1513 bp	1.0	91.1
matK-trnK-UUU_p2	TCGCGACGAGAATGGAATTT	TGACAATGGCGTCTCAAACA	45.0	45.0	58.0	58.0	1508-1671 bp	0.980769	90.9
matK-trnK-UUU_p3	TCGCGACGAGAATGGAATTT	TTGGCTCTTTCCTTCGCTTT	45.0	45.0	58.0	58.0	1545-1603 bp	0.961538	90.3
matK-trnK-UUU_p4	ACTAATCGCGACGAGAATGG	TTGGCTCTTTCCTTCGCTTT	50.0	45.0	57.9	58.0	1550-1608 bp	0.961538	89.3
matK-trnK-UUU_p5	TCGCGACGAGAATGGAATTT	ATCCCCATTCCCTATTGGCT	45.0	50.0	58.0	58.1	1445-2278 bp	0.961538	89.2