Hotspot

rpoBb

Genome · 26107-27747 · 1641 bp

Back to Coelastrum

Pi 0.165

MarkerSeek score 81.00

Primer available yes

Label rpoBb

Feature Metadata

label_name: rpoBb
feature_type: gene
region: Genome
length_bp: 1641
variable_sites: 492
pi: 0.165
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoBb_p1	TGGAGAAAAGGATTCGGCTT	TAATGCGTACTGAAAGGCCC	45.0	50.0	57.1	58.0	2359 bp	0.2	40.7
rpoBb_p2	TTGGAGAAAAGGATTCGGCT	TAATGCGTACTGAAAGGCCC	45.0	50.0	57.1	58.0	2360 bp	0.2	40.7
rpoBb_p3	TGGAGAAAAGGATTCGGCTT	TGCAAAGACAAGCAGTTCCT	45.0	45.0	57.1	57.9	2380 bp	0.2	40.5
rpoBb_p4	TTGGAGAAAAGGATTCGGCT	TGCAAAGACAAGCAGTTCCT	45.0	45.0	57.1	57.9	2381 bp	0.2	40.5
rpoBb_p5	TGGAGAAAAGGATTCGGCTT	GGTACTGGATTGGAAGCTCG	45.0	55.0	57.1	58.1	2333 bp	0.2	40.5