Hotspot

psbA

Genome · 67123-73738 · 6616 bp

Back to Coelastrum

Pi 0.353

MarkerSeek score 70.90

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: Genome
length_bp: 6616
variable_sites: 3266
pi: 0.353
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AGCCAGAGATAGCGATCAGT	AATGCACCCTTTCCACATGT	50.0	45.0	58.0	58.0	754 bp	0.2	47.8
psbA_p2	AGCCAGAGATAGCGATCAGT	ATGCACCCTTTCCACATGTT	50.0	45.0	58.0	58.0	753 bp	0.2	47.8
psbA_p3	GTGGAGAGGCACTATGAAGC	AATGCACCCTTTCCACATGT	55.0	45.0	58.1	58.0	861 bp	0.2	47.5
psbA_p4	GTGGAGAGGCACTATGAAGC	ATGCACCCTTTCCACATGTT	55.0	45.0	58.1	58.0	860 bp	0.2	47.5
psbA_p5	GTAACCGTGAGCAGCTACAA	ATGCACCCTTTCCACATGTT	50.0	45.0	57.9	58.0	1276 bp	0.2	47.3