Hotspot

cemA

Genome · 104484-105620 · 1137 bp

Back to Coelastrum

Pi 0.169
MarkerSeek score 82.50
Primer available yes
Label cemA

Feature Metadata

label_name
cemA
feature_type
gene
region
Genome
length_bp
1137
variable_sites
341
pi
0.169
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.96
markerseek_score
82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
cemA_p1 AACGAGCGCCTTAATCGAAT CTGGAGAAGCAGTCGCATT 45.0 52.6 58.1 57.9 2569 bp 0.2 38.3
cemA_p2 AACGAGCGCCTTAATCGAAT TGGAGAAGCAGTCGCATTG 45.0 52.6 58.1 58.2 2568 bp 0.2 38.2
cemA_p3 ATGCCTTGATTCGTCGTGAA CTGGAGAAGCAGTCGCATT 45.0 52.6 57.9 57.9 2506 bp 0.2 38.1
cemA_p4 ATGCCTTGATTCGTCGTGAA TGGAGAAGCAGTCGCATTG 45.0 52.6 57.9 58.2 2505 bp 0.2 38.0
cemA_p5 CTATGAGTGCCCAACCGAAA CTGGAGAAGCAGTCGCATT 50.0 52.6 57.9 57.9 2532 bp 0.2 37.9