Hotspot

trnG-GCC-rpoA

Genome · 142143-142771 · 629 bp

Back to Coelastrum

Pi 0.448

MarkerSeek score 73.10

Primer available yes

Label trnG-GCC-rpoA

Feature Metadata

label_name: trnG-GCC-rpoA
feature_type: igs
region: Genome
length_bp: 629
variable_sites: 360
pi: 0.448
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.54
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-rpoA_p1	GTAGGACTTGAACCCACGAC	GACCAACAAAACCAAACCTTCA	55.0	40.9	57.9	57.8	1216 bp	0.2	29.8
trnG-GCC-rpoA_p2	GTAGGACTTGAACCCACGAC	TCCAAGACCAACAAAACCAAAC	55.0	40.9	57.9	57.8	1221 bp	0.2	29.8
trnG-GCC-rpoA_p3	CTGTAGGACTTGAACCCACG	GACCAACAAAACCAAACCTTCA	55.0	40.9	57.9	57.8	1218 bp	0.2	29.8
trnG-GCC-rpoA_p4	CTGTAGGACTTGAACCCACG	TCCAAGACCAACAAAACCAAAC	55.0	40.9	57.9	57.8	1223 bp	0.2	29.8
trnG-GCC-rpoA_p5	GTAGGACTTGAACCCACGAC	CAAGACCAACAAAACCAAACCT	55.0	40.9	57.9	57.8	1219 bp	0.2	29.8