Hotspot

clpP-rpoC1

Genome · 118258-119865 · 1608 bp

Back to Coelastrum

Pi 0.437

MarkerSeek score 72.90

Primer available yes

Label clpP-rpoC1

Feature Metadata

label_name: clpP-rpoC1
feature_type: igs
region: Genome
length_bp: 1608
variable_sites: 906
pi: 0.437
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.55
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-rpoC1_p1	TTTAGCTGGTGGAACGATCG	GGAGACCAAATGGCAGTTCA	50.0	50.0	58.0	58.1	2641 bp	0.2	47.2
clpP-rpoC1_p2	TTTAGCTGGTGGAACGATCG	CCACTTTGCACCGATTAGGA	50.0	50.0	58.0	57.9	2748 bp	0.2	47.1
clpP-rpoC1_p3	CGCGTTCTCCAGCAAATTTT	GGAGACCAAATGGCAGTTCA	45.0	50.0	57.9	58.1	2924 bp	0.2	46.9
clpP-rpoC1_p4	TTTAGCTGGTGGAACGATCG	AGCAACAGGAGAGCCAATTC	50.0	50.0	58.0	58.2	2549 bp	0.2	46.6
clpP-rpoC1_p5	TTTAGCTGGTGGAACGATCG	GCAACAGGAGAGCCAATTCT	50.0	50.0	58.0	58.2	2548 bp	0.2	46.6