Hotspot

trnA-UGC-trnI-GAU

IRa · 150395-150538 · 144 bp

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Pi 0.0602

MarkerSeek score 79.60

Primer available yes

Label trnA-UGC-trnI-GAU

Feature Metadata

label_name: trnA-UGC-trnI-GAU
feature_type: igs
region: IRa
length_bp: 144
variable_sites: 14
pi: 0.0602
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnA-UGC-trnI-GAU_p1	CAACGACCCAATTGCAAGAG	TGATTTACTTCACGGGCGAG	50.0	50.0	57.7	58.0	177-241 bp	1.0	86.8
trnA-UGC-trnI-GAU_p2	CAACGACCCAATTGCAAGAG	AGGTCTCTGGTTCAAGTCCA	50.0	50.0	57.7	57.9	159-223 bp	1.0	86.5
trnA-UGC-trnI-GAU_p3	CCGCCTGGACAATTAGACAT	TGATTTACTTCACGGGCGAG	50.0	50.0	57.7	58.0	211-275 bp	1.0	86.0
trnA-UGC-trnI-GAU_p4	CCGCCTGGACAATTAGACAT	AGGTCTCTGGTTCAAGTCCA	50.0	50.0	57.7	57.9	193-257 bp	1.0	85.6
trnA-UGC-trnI-GAU_p5	AGACATCCAACCCGTAATCG	TGATTTACTTCACGGGCGAG	50.0	50.0	57.4	58.0	197-261 bp	1.0	84.2