Hotspot

trnI-GAU-trnA-UGC

IRb · 103952-104095 · 144 bp

Back to Codonopsis

Pi 0.0599

MarkerSeek score 75.00

Primer available yes

Label trnI-GAU-trnA-UGC

Feature Metadata

label_name: trnI-GAU-trnA-UGC
feature_type: igs
region: IRb
length_bp: 144
variable_sites: 14
pi: 0.0599
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.69
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-GAU-trnA-UGC_p1	TGATTTACTTCACGGGCGAG	CAACGACCCAATTGCAAGAG	50.0	50.0	58.0	57.7	177-241 bp	1.0	86.8
trnI-GAU-trnA-UGC_p2	AGGTCTCTGGTTCAAGTCCA	CAACGACCCAATTGCAAGAG	50.0	50.0	57.9	57.7	159-223 bp	1.0	86.5
trnI-GAU-trnA-UGC_p3	TGATTTACTTCACGGGCGAG	CCGCCTGGACAATTAGACAT	50.0	50.0	58.0	57.7	211-275 bp	1.0	86.0
trnI-GAU-trnA-UGC_p4	AGGTCTCTGGTTCAAGTCCA	CCGCCTGGACAATTAGACAT	50.0	50.0	57.9	57.7	193-257 bp	1.0	85.6
trnI-GAU-trnA-UGC_p5	TGATTTACTTCACGGGCGAG	AGACATCCAACCCGTAATCG	50.0	50.0	58.0	57.4	197-261 bp	1.0	84.2