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rps2-clpP

LSC · 74350-75831 · 1482 bp

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Pi 0.259

MarkerSeek score 76.80

Primer available yes

Label rps2-clpP

Feature Metadata

label_name: rps2-clpP
feature_type: igs
region: LSC
length_bp: 1482
variable_sites: 769
pi: 0.259
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-clpP_p1	CCGCATCTCTTTTCGGAAGA	AAGCAACGGAGCCATCATAG	50.0	50.0	58.0	58.0	1761-2654 bp	1.0	91.6
rps2-clpP_p2	AATGACACCGAGCGCTTATT	AAGCAACGGAGCCATCATAG	45.0	50.0	58.0	58.0	1629-2522 bp	1.0	91.5
rps2-clpP_p3	TTCGTTAACATACCGCTGGG	AAGCAACGGAGCCATCATAG	50.0	50.0	58.0	58.0	1703-2559 bp	0.777778	82.7
rps2-clpP_p4	CCGCATCTCTTTTCGGAAGA	GTATCGCCCAACCCTGTATG	50.0	55.0	58.0	58.1	1406-2264 bp	0.444444	68.7
rps2-clpP_p5	TTCGTTAACATACCGCTGGG	GTATCGCCCAACCCTGTATG	50.0	55.0	58.0	58.1	1311-2169 bp	0.444444	68.7