Hotspot

psaJ-rpl33

LSC · 69697-70144 · 448 bp

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Pi 0.0339

MarkerSeek score 73.90

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 448
variable_sites: 41
pi: 0.0339
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTTGGGAAATGCTCGGGAA	TAACACCCTTTCGCACACAA	45.0	45.0	58.0	58.0	842-932 bp	0.777778	75.5
psaJ-rpl33_p2	TTTTGGGAAATGCTCGGGAA	TTAACACCCTTTCGCACACA	45.0	45.0	58.0	58.0	843-933 bp	0.666667	69.5
psaJ-rpl33_p3	TTTTTGGGAAATGCTCGGGA	TAACACCCTTTCGCACACAA	45.0	45.0	58.0	58.0	843-933 bp	0.666667	69.5
psaJ-rpl33_p4	TTTTTGGGAAATGCTCGGGA	TTAACACCCTTTCGCACACA	45.0	45.0	58.0	58.0	844-934 bp	0.666667	69.5
psaJ-rpl33_p5	TCGATTTCACCGAATCCACC	TAACACCCTTTCGCACACAA	50.0	45.0	58.0	58.0	1178-1268 bp	0.444444	57.8