Hotspot

trnW-CAA-trnP-UGG

LSC · 68907-69083 · 177 bp

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Pi 0.0381

MarkerSeek score 73.00

Primer available yes

Label trnW-CAA-trnP-UGG

Feature Metadata

label_name: trnW-CAA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 177
variable_sites: 19
pi: 0.0381
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CAA-trnP-UGG_p1	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	50.0	50.0	58.0	58.1	251-266 bp	1.0	86.7
trnW-CAA-trnP-UGG_p2	TGCCGAACTGAACTAAGAGC	ATTGAAGGGATGTAGCGCAG	50.0	50.0	57.9	58.0	272-287 bp	1.0	86.2
trnW-CAA-trnP-UGG_p3	GTTTTGGAGACCCACGTTCT	ATTGAAGGGATGTAGCGCAG	50.0	50.0	58.0	58.0	291-306 bp	1.0	86.1
trnW-CAA-trnP-UGG_p4	TTTTGGAGACCCACGTTCTG	ATTGAAGGGATGTAGCGCAG	50.0	50.0	58.0	58.0	290-305 bp	1.0	86.1
trnW-CAA-trnP-UGG_p5	ACCTACGACATCGGGTTTTG	ATTGAAGGGATGTAGCGCAG	50.0	50.0	57.9	58.0	305-320 bp	1.0	85.4